● Description
Spin Column type product that design for isolate or purify, over 20ug high purity Plasmid DNA
· High reproducibility of over 20ug high yield and purified plasmid DNA
· Suitable isolation and purification for over 30kb
· Lysis & Neutralization indicator (Lysis Viewer) applied
DNA-Spin™ Plasmid DNA Purification Kit has optimized alkali lysis buffering system and cilica membrane to isolate high purify plamid DNA from bacteria. Neutralization buffering system applied for neutral pH and binding.
It makes not only stable isolation and purification but also efficient
to remove chromosomal DNA, cell debris of plasmid DNA for over 30 Kb. Lysis Viewer is applied to visual pH confirmation as lysis & neutralization indicator, so you can easily find the pH condition during the alkaline lysis step (Fig 1.). High-copy plasmid DNA and low-copy
plasmid DNA are all available to use. Washing Buffer A is separately
offered for efficient isolation and purification of plasmid DNA from endA+ strain(HB101, the JM strains, NM series strains, PR series strains).
● Description
Effect of charging condition by Advanced Sephadex Resin makes yield maximized. Only two times of spin down can purify labeled probe DNA.
· Maximized yield by advanced Sephadex Resin
Efficient for small quantity labeled probe DNA
Optimized for small size(100 bp ~ 1 Kb) labeled probe DNA purification
Effective removal of dNTP, free isotope and chemicals after Probe DNA labeling
· Only two times of Spin down can complete purification
PROBER™ Probe
DNA Purifying System is disposable column, which is designed for
un-incorporated nucleotide removal after DNA labeling during react of
polymerization or end-labeling, random primer labeling, nick translation
using sephadex.
Sephadex pillar is build up by the spin down using charged sephadex resin. You can get labeled probe DNA if load the sample in the sephadex pillar and perform spin down. Especially, our advanced sephadex resin makes effective removal of dNTP, free isotope, enzyme after probe DNA labeling step by chemical method or isotope. Two times of spin down step is enough for simple and high probe DNA reproducibility.
● Applications
· Purification after random primer labeling
· Purification after Nick translation or polymerization
· Purification after react End-labeling
· 100 bp ~ 1 Kb labeled probe DNA purification etc
● Description
DNA-maxi SV Kit is designed for easy and fast maxi scale plasmid DNA isolation and purification.
§ Spin and vacuum type kit designed for efficient isolation and purification with Maxi Scale Sample.
High copy number : 100~150 ml culture volume
Low copy number : 300~400 ml culture volume
§ Takes 40 minutes to extract high yield plasmid DNA.(vacuum method)
§ Pre-filtration column applied to remove Cell lysate.
§ Centrifugation method(Swing bucket rotor only) makes Ethanol settlement needlessness.
§ Simple and stable plasmid DNA isolation and purification with over 30 Kb.
§ Lysis & Neutralization indicator (Lysis Viewer) applied.
DNA-maxi™ SV Plasmid DNA Purification Kit is designed to extract high purity /concentration plasmid DNA from midi scale bacterial cell culture volume by using silica membrane and optimized alkali lysis
buffering system. Two column system helps easy isolation and
purification with pre-filtration column and DNA binding membrane applied
binding membrane. So you can extract and purify DNA within 40min. using
the vacuum method (Fig 1. Combination
of Pre & Binding Column). Neutralization buffering system affects
pH neutralization and binding for stable extraction and purification
from over 30Kb size plasmid DNA. Effective chromosomal DNA, cell debris removal is also possible with that system. Lysis Viewer is applied to visual pH confirmation as lysis & neutralization indicator, so you can easily find the pH condition during the alkaline lysis step. High-copy plasmid DNA and low-copy
plasmid DNA are all available to use. Washing Buffer A is separately
offered for efficient isolation and purification of plasmid DNA from endA+ strain(HB101, the JM strains, NM series strains, PR series strains).
● Application
§ Ligation & transformation
§ PCR & Real-time PCR
§ Automated fluorescent sequencing
§ Transfection
§ Restriction digestion etc.
