Home » DNA-maxi™ SV Plasmid DNA Purification Kit
DNA-maxi™ SV Plasmid DNA Purification Kit
| Cat.No | Capacity | Price | Order | Shopping cart | |||||
| 17253 | 12 Col. | INQUIRE |
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● Description
DNA-maxi SV Kit is designed for easy and fast maxi scale plasmid DNA isolation and purification.
§ Spin and vacuum type kit designed for efficient isolation and purification with Maxi Scale Sample.
High copy number : 100~150 ml culture volume
Low copy number : 300~400 ml culture volume
§ Takes 40 minutes to extract high yield plasmid DNA.(vacuum method)
§ Pre-filtration column applied to remove Cell lysate.
§ Centrifugation method(Swing bucket rotor only) makes Ethanol settlement needlessness.
§ Simple and stable plasmid DNA isolation and purification with over 30 Kb.
§ Lysis & Neutralization indicator (Lysis Viewer) applied.
DNA-maxi™ SV Plasmid DNA Purification Kit is designed to extract high purity /concentration plasmid DNA from midi scale bacterial cell culture volume by using silica membrane and optimized alkali lysis
buffering system. Two column system helps easy isolation and
purification with pre-filtration column and DNA binding membrane applied
binding membrane. So you can extract and purify DNA within 40min. using
the vacuum method (Fig 1. Combination
of Pre & Binding Column). Neutralization buffering system affects
pH neutralization and binding for stable extraction and purification
from over 30Kb size plasmid DNA. Effective chromosomal DNA, cell debris removal is also possible with that system. Lysis Viewer is applied to visual pH confirmation as lysis & neutralization indicator, so you can easily find the pH condition during the alkaline lysis step. High-copy plasmid DNA and low-copy
plasmid DNA are all available to use. Washing Buffer A is separately
offered for efficient isolation and purification of plasmid DNA from endA+ strain(HB101, the JM strains, NM series strains, PR series strains).
● Application
§ Ligation & transformation
§ PCR & Real-time PCR
§ Automated fluorescent sequencing
§ Transfection
§ Restriction digestion etc.
